A pipette filter can prevent cross-contamination by preventing aerosol and liquid contaminants from reaching the pipette tip. These filters are FDA-compliant and made from proprietary Vyon porous plastic. Their high porosity allows them to solve some of the common challenges of traditional pipette tips. Here are some benefits of pipette filters. These products are an affordable and environmentally friendly alternative to filters for pipettes.
Pipette filters are ideal for small volumes. They absorb a significant amount of material. Pipette filtration is particularly useful for GC and NMR experiments, which require liquids without suspended solids. Filters can be made with long rods that can be wedged into a Pasteur pipette. Large septa can hold a GC vial securely. Using a pipette filter ensures a solid-free sample.
The main advantage of pipette filters is that they prevent cross-contamination. Standard pipette tips can potentially contain aerosols and may lead to cross-contamination. Filter tips eliminate this risk by removing aerosols from the tip. They also prevent the formation of aerosols, which can remain in the air for extended periods and cause infections. Hence, filter tips are the most practical solution for sensitive samples.
A pipette tip with a filter prevents cross-contamination and gives more robust results. In addition to this, a filter tip acts as a training wheel for new lab members. If a new member accidentally aspirates liquid or reagent into the pipette, it is usually too late to discard the entire pipette. However, a filter tip can be replaced much easier than sending the entire pipette to the lab for repair.
You may ask: why can't I get everything out of my pipette tip? The answer is that the tip you are using is not suitable for the liquid you are trying to collect. Using the wrong type of pipette tip can completely ruin your accuracy. The wrong tip can lead to a waste of precious samples and reagents, and even repetitive stress injuries. Furthermore, incorrectly used tips may not fit the pipettes. It is best to buy a pipette with a high-quality tip to ensure proper performance. If you're not sure about the tip, you can try the right one in a gravimetric test.
There are three basic types of pipette tips: non-sterile and sterile. These are used most frequently in non-sensitive applications such as loading agarose gels or isolating DNA. While these are cheaper alternatives, the bulk tips and reloads aren't sterile. To maintain the sterility of your pipette tips, you can sterilize the pipette storage box with an autoclave.
When it comes to filtration, tips can make or break a sample. High-quality tips should be virgin polypropylene, which means they are free of metal or plastic additives. You should also check the material composition of colored tips to ensure that they are free of dyes or metal additives. In addition, high-quality tips should be sterile and non-reactive, which means that they won't transfer contaminants and contamination from samples to the tip.
When choosing a filter tip, the manufacturer must validate the sterilization process and conduct regular quality checks. While individual laboratories rarely test their autoclaving process, the manufacturers of filtered tips perform quality checks on their products. Autoclaved tips are sterile, free of RNase, and free of any living organisms. This is important because sensitive assays require sterile pipette tips. Pipettes also create aerosols that can potentially contaminate samples, and filter tips prevent this from happening.
Filtered tips are useful for sensitive assays, such as PCR. These tips prevent liquid splashing and minimize aerosol penetration during pipetting. They are also ideal for low-volume applications. Filtered tips come in two types: standard filter tip and self-sealing barrier tip. Both are made to prevent cross-contamination. To protect against cross-contamination, filtered tips are generally more expensive than standard pipette tips.
Filtered pipette tips also have an advantage over regular pipette tips. They protect the pipette from aerosols and other harmful particles. Additionally, they prevent cross-contamination, which can lead to compromised results. Because filtered tips have a liquid-sealing barrier, they are ideal for sensitive applications. They are also nonpyrogenic and pre-sterile. A sterile pipette tip is an essential component of sensitive assays.
Different pipette tips have different performance requirements. Using the wrong tip will not only contaminate samples, it will also waste reagents. It may even cause repetitive stress injury if the wrong tip is used. Using the wrong type of tip can also affect the accuracy of your measurements. Purchasing the correct tip for the application is essential for accurate and repeatable results. The GLP Guide provides an explanation of the various types of pipette tips.
When choosing the correct pipette tips, you should look for a tip that fits snugly in the pipette barrel. If the tip is not a snug fit, air will escape from the pipette barrel, which will lead to an error in aspirating the proper volume of liquid. Filtered tips come in three main categories: general and filtered. For the general pipette, the latter one is preferred.